Pharma Operations

Pharma Operations

What is the difference between Data and Information?

Most of the cases we think that “data” and “information” are same but they actually aren’t the same though they are often used interchangeably. There are elusive differences between these two components & their purposes of use. information is organization & interpretation of group of facts where Data is defined as individual facts. To identify and solve problems, you can use the data & information together. To drive a successful business, we can use these two components to accelerate the ultimate mission to reach the goal.

What Is Data?

Collection of individual facts or statistics is defined as data (Data is plural form of ”datum” but the term didn’t use in for daily expression. Data has its various type of form such as figures, text, observations, numbers, images, graphs, or symbols. Individual dates, prices, weights, addresses, ages, temperatures, distances, names, etc. can the example of data.

Data is simply defined as “facts & figures”. Each piece of data is a tiny fact that doesn’t mean abundant of its own. Data can be defined for singular fact or collection of facts. It comes from the Latin word ”datum”, mean “something given”. “datum” is technically correct singular form of data but is hardly used in public language. Its early usage dates back to 1600s. Over time “data” has become plural of “datum”.

Data doesn’t carry any significance or purpose, it’s the raw form of knowledge. To make it meaningful you have to interpret data. Bits & bytes are used to measured data which are units of information in context of computer storage & processing the same.

Data without analyzed, organized, and interpreted may even seem useless & data can be simple. Two types of data are depicted here-
[][]Quantitative data is in numerical form, like volume, weight, cost of an item. Its not descriptive.
[][]Qualitative data is descriptive, like sex, name, or cloth color of a person. It’s not but non-numerical.

What Is Information?

It can be defined as act of knowledge gaining process through research, study, communication, or instruction. Information is the totality of group of analyzed and interpreting data. A data is always the individual numbers, figures, or graphs whereas information is considering the perception of those items. In this era, we can mention that most of the sophisticated modern industry always maintain environmental monitoring through recording of Temperature & Relative Humidity, through out the year of the year and achieve it in a suitable position.

Information can be defined as “news or knowledge received or given”. Processed, interpreted & organized facts is information. It comes from the Latin word īnfōrmātiō, mean “formation or conception.”

This type of recoding doesn’t bear any significant meaning but if you organize, analyze the recoded data then you can easily realize the Environmental condition changes in specific season. You can trend the data to sort out the best matching, minimum maximum data etc. which useful to set up or install the best quality BMS [Building Management System] parameter. Without analyzing and organizing the data, it is the just piece of recording doesn’t denote any significant value. A well-organized data can help the others.

In basic terms, it can be concluded that data is unorganized explanation of raw facts from which information can be take out.

Significant Differences Between Data vs Information

[][]collection of facts is considered as Data where information puts all of those facts into context.
[][]Data is always raw & unorganized where information is processed and organized.
[][]Data points are individual & most of the time it is unrelated. Information relates these points and show the actual behind it.
[][]Without analyzed and interpretation data is totally meaningless, when it organized then it became meaningful information.
[][]Data is always independent but Information depends on data due to you can’t get any information without processing data.
[][]Numbers, graphs, figures, or statistics is the form of data. Information generally appears as language, words, thoughts, ideas etc.
[][]To base on data, you can’t make any decision but when information available at your hand you can make any decision. So, data are not enough to make any decision, information require to do the same.
[][]Data always defines figures & facts. It comprises of one entry or collection of diverse values. Information defines values & context together, resulting in approximately meaningful. It forms an organized & interconnected structure, from data, to interpret or link the whole.

[][]Example
=>For data examples, we can use Lance, M. Kiely, 4590 Neville Street, Terre Haute, IN 47807. The separator [commas] characterize each distinct fact that may or may not be linked to others.
=>In this example of information, Each fact narrates to other facts to form a concept, known as Lance M. Kiely. Creating this Lance M. Kiely entity allows people to reason, calculate, & do other influences.
Lance M. Kiely
4590 Neville Street
Terre Haute, IN 47807

Data vs. Information in Computers

If we consider computers, Data can be considered as INPUT on anything that instruct to computer to do or store. The OUTPUT of the computer which exhibit your computer after your instruction to computer.

As per statistics, data defined as raw information but term statistics is often used in place of information. Statistics interpret & summarize data.
In business, data are often raw numbers & information is a collection of separate data points which you use to realize what you’ve restrained.

[][]1.0
Data: typing the words “Dog videos” in your computer web search engine (INPUT).
Information: The list of search results which includes a variability of dog videos on the resulted browser page (OUTPUT).

[][]2.0
Data: 9994565566
Information: phone number (555)456-5566 of a person.

[][]3.0
Data: 46.07 & 789
Information: Molar mass & Density of Ethanol in g/mol & kg/m³

[][]4.0
Data: 70%
Information: Isopropyl alcohol in percentage

[][]5.0
Data: -16
Information: Freezing points of Vodka in Fahrenheit

Difference Between Data and Information

ParametersDataInformation
ContentUnrefined raw factors.Refined in a meaningful way.
CharacteristicData is considered property of a specific organization & is not offered for sale in the public.Information is offered for sale to public.
Decision MakingRaw data is insufficient to make any decision.Information is enough to make any decision.
DependencyData depends upon the sources for collecting method.Information always depends upon data.
Design of Data Data is never designed for specific need of user.Information is always explicit to requirements & expectations because all extraneous facts & figures are detached, during transformation process.
Dependency levelData never depends on Information.Information constantly depended on Data.
DescriptionHelps to develop ideas or conclusions based on Qualitative or Quantitative Variables.It is group of data which carries news and meaning.
EtymologyData has comes from Latin word, datum, means “To give something.” The word “data” become plural of datum.It comes from the Latin word īnfōrmātiō, mean “formation or conception.”
Example1.0 During word Tour Ticket sales on a specific Band.1.0 Sales report generate by region & venue gives information which venue perform best.
Example2.0 An example of data is a student’s Eye Color.2.0 The average Eye Color of a class is the information derived from the given data.
FormatData found in the form of letters, numbers, or a set of characters.Ideas and inferences
FeatureData is a single unit & raw. It doesn’t have any meaning alone.Information is artefact & group of data which jointly carry a logical meaning.
InterrelationCollected Information.Processed Information.
Knowledge levelLow-level of knowledge.Second level of knowledge.
MeaningData does not have any definite persistence.It conveys meaning that has been allocated by interpreting data.
Measuring unitMeasured in bits & bytes.Measured in different meaningful unit like time, quantity, etc.
Meaning of baseData is based on records & explanations and, which are deposited in computers or remembered by a individual.Information is considered more consistent than data. It helps investigator to conduct a appropriate analysis.
Support for Decision makingData can’t be used for decision makingIt is extensively used for decision making.
SignificanceData collected by the researcher, may or may not be useful in different situation.Information is useful & appreciated as it is readily accessible to the researcher for use.

List of Examples of Data vs Information

differences between data and information, how these examples turn data into insights:

[][]An individual customer’s bill amount is data at a specific restaurant but after a certain period of time or after one day collection when the restaurant Manager or owner collect all the customer bill of that day or time, it can produce valuable information of the restaurant as it can produce which item of the restaurant is hot cake or what item is running well and what are not. After that the restaurant, can realize how they can maintain the inventory of a specific item and how to continue their service as well as to minimize the overhead, wadges, supplies etc.

[][]An individual customer service survey of a restaurant is a data but after a period of time when compile the all the survey, then it can produce valuable data regarding area of improvement of the restaurant such as customer service, price, cleaning, mannerism, hospitality, space, location, viewpoint etc.

[][]A single social media like on a media post is a data but when multiple social media item like comments, share, statistics etc. are compiled then the specific company can focus on the specific social media where they are performing best and where they are in worst condition. Comments from a social post of multiple social media is very useful to do the same. It helps the company to set their goal based on the comments collect from customer and it help to find out multiple idea from multiple customers.

[][]On their own, inventory levels are data. However, when companies analyze and interpret that data over a range of time, they can pinpoint supply chain issues and enhance the efficiency of their systems.

[][]Inventory management of the company for the different item is the data but when it collects for certain period of time it can be valuable information regarding the inventory item which can help the supply chain management system to run their activity appropriately.

[][]A Price of a specific item is a valuable data but when processing the data from multiple company can produce valuable information regarding market gap, advantage of the competitor, profit margin, bonus, discount, policy etc. for the specific item.

[][]Taste of Azithromycin Suspension is a data but when you collect different taste from different company product you can produce valuable information regarding taste that which taste is more acceptable to the end user i.e., mango/orange/strawberry/pineapple etc. from this activity you can collect valuable information and implement the same for your company product.

[][]Temperature readings all over the world for the past 10 years can be consider as data. When this data is organized, analyzed to find out global temperature condition is raising over the period of time, then this data changed to information.

[][]Number of visitors to a specific website by country of the word is an example of data. Finding out that the traffic source from Canada is decreasing while that from Austria is increasing is meaningful information.

[][]Often data essential to back up a claim or supposition consequent or inferred from it. Such as before a drug is approved by FDA, manufacturer must conduct clinical trials & must have submit lot of data to reveal that the drug is safe.

“Misleading” Data

[][]Due to the processing of data, interpreted & analyzed, this is very possible that it can be interpreted incorrectly. When this leads to specious conclusions, it can be said that data are misleading. Often this is the consequence of imperfect data or a lack of framework. Such as your investment in a mutual fund may be up by 7% & you may accomplish that fund managers did a great job. Nevertheless, this could be misleading if major stock market indices are up by 10%. In this case, the fund has floundered the market pointedly.

[][]In the year of 2007, Famous toothpaste company Colgate ran an ad campaign & stating that 80% of the dentists recommend Colgate Toothpaste for safe dental health. From this promotion, many consumers assumed that Colgate was the best choice for their safe dental health for daily use. But in practical, this wasn’t inevitably true. In reality, this is the well-known example of misleading data & information.

[][]Anchor Tucker Carlson presented a graph saying, number of Americans recognizing as Christians had distorted over last decade during one of Fox News’s broadcasts. Over the image above, a graph showing in 2009, Christian Americans is 77%, number decreased to 65% in the year of 2019. Now, if issue here is not noticeable enough, here the Y-axis in that chart starts from 58% & ends at 78%, making the 12% drop from 2009 to 2019 look way more substantial than it really is.
Sample size is the vital point to make any key decision for the organization. Making any decision data collected from 100 sample is more accurate data collect from 10,000 sample. Data collect from 100 sample is misleading compare to 10,000. A key decision shall be make from vast amount of sample.

[][]Federal Trade Commission (FTC) filed a lawsuit against car company Volkswagen , which claimed that car company had betrayed customers with advertising campaign it used to promote its allegedly “Clean Diesel” vehicles, according to a press release.

In the year of 2015, it was uncovered that Volkswagen had been cheating emissions tests for its diesel cars in US in the past 7 years. The Federal Trade Commission, alleged that “Volkswagen cheated consumers by selling or leasing more than 550,000 diesel cars based on the false claims that cars were low-emission & environmentally friendly.” For their false claim, the company was remarkably fine up to $61 billion for the violation of Clean Air Act.

[][]Red Bull, Energy drinks company was sued in 2014 their slogan “Red Bull gives you wings.” The company settled case by agreeing to pay out maximum of $13 million — including giving $10 to every US consumer who had bought their drink since 2002.

They claim that the caffeinated drink could improve consumer’s concentration & reaction speed; the tagline company use for last two decade went alongside marketing claims. One of the regular customers of Red Bull drink claim that that he had not developed “wings,” or shown any signs of enhanced intellectual or physical capabilities.

[][]In 2010, Kellogg’s widespread Rice Krispies cereal had a crisis when it was defendant of misleading consumers about product’s immunity-boosting properties. The Federal Trade Commission [FTC] ordered Kellogg to close all advertising which claimed, cereal enhanced a child’s immunity with “25 percent Daily Value of Antioxidants and Nutrients -Vitamins A, B, C and E,” affirming the claims were “dubious.”

[][]New Balance, the famous show making company [Owner, Jim Davis, own almost 95% total share of this company] was defendant of false advertising in 2011 over a sneaker range which claimed that it could help wearers to burn calories but it was subsequently found that there were no health assistances from wearing this sneaker range. From New Balance, they explain that using hidden board technology & it was advertised as calorie burners which activated the quads, glutes, hamstrings & calves. New Balance agreed to pay a settlement of $2.3 million on August 20, 2012.

How Businesses Can Leverage Data & Information

Is it come to the point to distinction between data vs information really matter for businesses? If any company that company collect accurate data then interpreting it and generate information and implement the same on right time on right place can realize the actual benefit for the company.
For example, a company might gather data about the performance of their ads or content. Running a successful add or content to the various platform can produce valuable data. From the data they can produce right information regarding product design, brochure generation, promotional activity, product awareness, customer demand and customer buying capacity.

This can also help to develop target customer, future offering, promotion, branding and developing multiple products for the company.
Right data can lead the organization to the right goal but to maintain the right set of data is very difficult. There are several blockades to create a data dependent better smart organizational culture. Different team of an organization may collect & maintain disparate sets of information. Hence a central database system is crucially need for the organization. Without a central database system, none one can earn the actual benefit and interpretation of data may fail. Data need to supervise by someone, without proper supervision data may not maintain its proper quality and generate poor data mislead the organization.

Any business depends on expressive data patterns to get information. There are dissimilarities between data and information. Business relies on meaningful data patterns to get information, in this article let’s explore the differences and similarities between data and information. Misinterpretation the difference between “data” & “information” sets up the stage for slip-ups. Like the six blind men in an Indian legend, trying to define an elephant, end up puzzling discrete facts, or data, as information or meaning.

In six blind men’s dilemma, individually complicates data (trunk or legs) for information (an elephant is like giant cow or an elephant is like a giant snake). Likewise, anyone can collect customer data & think they have the full customer information when they are actually not. Data & Information have specific implementation. To correctly recognize & use either one, you need to understand the change between data & information is.

To create an effective data driven organization, then you need to maintain the data source which must available across the group of qualified people who are technically sound to generate information from processed data maintaining appropriate protocol to assure the proper data quality.
Data is very critical to generate information and both these two items is crucial to make any decision for the organization.

DIKW [Data Information Knowledge Wisdom] Model

DIKW is the model used for discussion of data, information, knowledge, wisdom & their interrelationships. It denote functional or structural relationships between data, information, knowledge & wisdom.

Are data and information the same thing?

Data is based on observation & records which frequently store in computers or simply memorize it by individual. On the other hand, information denotes to be more consistent than data. In other words, it is a proper analysis which researchers or investigators conduct for converting data into information.

Data and information may be the same thing, From a content & format perspective. For example, you can point same values in two diverse columns on a spreadsheet. Nevertheless, data & information contents & formats do not have to match. In any case, you use data & information very in a different way.
If you want to sort out the value “New York, United States” You will filter data named “New York” under city and “United States” under country.

On the same spreadsheet, If you want to know if the Lance M. Kiely records mean the identical person. Then look at the information in both rows & see, across the columns:
Lance M. Kiely
4590 Neville Street
Terre Haute, IN 47807
You determine both Lance M. Kiely, living in New York, United States, mean the same customer thing from the information provided.

How do data and information differ?

Though Data & information may have the same values but from the creation & business usage they may differ. Data generally includes entries whereas Information contains context. Information comprise data with different contents & formats & be the same thing.

As per data perspective point, “United States,” “UNITED STATES,” and “U.S.A.” represent entirely different facts based on number of characters & formatting varies. Therefore, Lance M. Kiely, who lives in U.S.A., is not same customer as Lance M. Kiely, who lives in United States.

If we consider information viewing platform, the “United States,” “UNITED STATES,” & “U.S.A.” represent the same thing for geographical reason because someone with understanding of geography can point to the “United States” or the “U.S.A.” on a American Map.

The correct data and accompanying context make the United States and the U.S.A. contain meaning about a shared concept of that region, like culture, sports, and government. From the shared concept of that region, like culture, sports, and government make the data more accurate. Lance M. Kiely, who lives in U.S.A., with Lance M. Kiely lives in the United States, and consider creating the same object. Comparing with the other people lives in United States using additional data points like cultural activities in U.K.

Frequently Asked Questions

What is data? Explain with example.

Raw, unorganized, unprocessed facts are known as Data. All of the facts consider as data until it processed, organized such as all information writing on the paper is data until its processed & organized in suitable manner.

What is information?

Processed, organized data which is advantageous in providing useful facts is known as Information. For Ex. It can be concluded that if data are processed and organized in right way generate valuable piece of information.

What is valid information?

A reliable fact is considered as Valid information. Checked & verified information that is ready for use in a specific purpose.

What is the classification of Data?

Classification of Data

Data classification is a critical element of any information security & compliance program, especially if any organization stores big volumes of data. To understand the data security strategy, classification of data plays an important role providing information that where the sensitive data shall be stored. It provides valuable information regarding unused data & elimination of the same type reduce the maintenance cost for the organization.

Types of Data Classification

[][]Content-based classification inspects & interprets files to classify sensitive information.
[][]Context-based classification looks at location, application, creator tags & other variables as secondary indicators of subtle information.
[][]User-based classification depends on manual selection of each document by an individual.

Basic Classification Scheme

The modest scheme is three-level classification:

[][]Public data
Data that can be freely revealed to the public. Examples include any company contact information & any browser cookie policy.

[][]Internal data
Data that has low security level but is not for public expose, like marketing research for a product.

[][]Restricted data
Highly subtle internal data. Expose to public platform create negative impact on operations and put the company at financial or legal risk. Restricted data entails the highest level of security protection at any cost.

Government Classification Scheme

Government agencies use three levels of sensitivity as top secret, secret and public but based on situation can be classified into five types

[][]Top secret-Cryptologic & communications intelligence
[][]Secret-Selected military plans
[][]Confidential-Data signifying the strength of ground forces
[][]Classified-Data labelled “For Official Use Only”
[][]Unclassified-Data that may be publicly released after authorization of respective body.

Commercial Classification

Typically, organizations that store & process commercial data use 4 levels to classify data: 3 private levels and one public level.

[][]Sensitive- Intellectual property, Secrete Formulation, PHI
[][]Confidential-Vendor contracts, employee reviews, Contract, Special Allowance
[][]Private-Customer names or images, Sensitive Video promotion
[][]Proprietary-Organizational processes, Quality System
[][]Public-Information that may be disclosed to anyone

What is the meaning of the two types of data?

The two types of data are qualitative & quantitative. Qualitative data is non-numerical data like eye color, skin texture, Hair color, Shoe color, Clothing color and more. On the other hand, quantitative data is in the form of numbers like the weight of books, number of apples, number bird and more.

What is the difference between Data and Information? Read More »

Forced Degradation Study or Stress Testing Procedure

What is Degradation?

Forced Degradation Study before proceeding on it ,first of all  ”Degradation’‘ to be discuss first, This is the act of lowering to some degree or someone to a less respected state or position. A CEO of a multinational company resigning from his office is a degradation. It’s also a downcast state.

The word degradation is very much related to the degrade, which comes from Latin word Degradare. The word “Degradare” comes from “de”-, meaning “down,” & gradus, meaning “Step.” So, it is very much clear that the degradation as a step down, or feeling as though you’re a step below.

Degradation products

It is the unwanted chemicals which can generate during manufacturing, transportation & storage of pharmaceutical drug products & can affect efficacy of pharmaceutical drug products. A small amounts of pharmaceutical degradation products can affect crucial safety because of the potential to cause adverse effects in end user.

Subsequently, it is crucial to focus on formulation, storage conditions, transportation, distribution channel and packaging to prevent the formation of degradation products which can negatively affect quality, safety and efficacy of the pharmaceutical drug products.

To find out the main cause of degradation of the pharmaceutical product is the crucial point, various software and data tracking system can help in this matter. This system can provide useful information during transportation and storage of pharmaceutical products, the route shall be determined to estimate the main cause.

Presence of a genotoxic degradation product shall tend to more assessment if it identified on due time. The chemical structure of the substance shall be determined to identify the toxic alerting structures associated toxic products, products [Compound] without active structure is marked as ordinary impurities.

A risk/benefit analysis shall be done to evaluate the levels of degradation products and most of the nest pharmaceutical call its mandatory. During the development of any type of product either critical or non-critical drug, critical variable of the drug products shall be follow-up which will control the degree of degradation of impurities.

Now a days the impurity profile has been considered as the key point of the product quality. It is the essential part of the quality parameter for the various competent regulatory authority. The toxicological evaluation and impurity profile become the key point of the degradation products to confirm its certain level of efficacy. Various types of test method have been identified to investigate the degradation products, all of them assay method consider the best to all and it’s highlighted to prove its effectivity.

The purity, safety and efficacy of the product depend on the stability of the product and it is the critical parameter of all the parameter. A product must be stable at a certain period of time to prove its efficacy, potency and safety.

A less stable or changes of stability can create serious toxicological effect by forming toxic degradation products and deliver less active or less effective or less potent drugs to the end user. Under these circumstances, this is very crucial to known the actual behavior of the drug products in various surrounding or environmental conditions.

Dissolution test are considering the most quality control tool for the commercial batch to batch product to monitor its consistency over a certain period of time. It also provides significant information during post approval changes of the certain product as changes made in formulation, manufacturing process and different scale up procedure.

To confirm the quality, safety and efficacy, the chemical stability is very important for a pharmaceutical product. This is very important to know the environmental influences of a certain developed product in specific condition such as Heat [Temperature], Humidity [Relative Humidity] & Light [Photostability] and this also regulatory [ICH & FDA] requirements.

Data acquire from stability study denote the shelf life and storage condition of the specific tested drugs, the container closure system [Protective packaging system] also require to satisfy the regulatory expectation.

Different types of method/instruments are available to determine the degradant compounds which are readily present during the forced degradation study period. HPLC-UV [HPLC with UV detector] and HPLC-PDA [ HPLC with Photodiode Array Detector] is the renowned method and extensively used in pharmaceutical company at the time of degradation study and validation and development of various type of method.

LC-MS [HPLC with Mass Detector], GC-MS [Gas Chromatography with Mass Detector] and NMR [Nuclear Magnetic Resonance] spectroscopy are significant methods to detect the degradants’ structure.

What is Forced Degradation Study?

Exposure of specific sample at the unfavorable/stress condition of Heat [Temperature], Humidity [Relative Humidity], Light [Photostability], Oxidation and Acidic/Basic condition; observe/detect the changes of those sample or measure the rate of changes/degradation, mainly in Efficacy, Safety and Potency parameter of drug substance. Forced degradation study is the key point during the development of a specific drug. Determination of the type changes denote the modification or changes of the development process.

Now a days Force Degradation Study become the prerequisite to submit the NDA to regulatory authority and it became the quality parameter for the new product. During the regulatory submission, the Force Degradation Study data shall be submitted to get satisfactory result from FDA. Some of the best application of Force Degradation Study is depicted here-

[][]Developing and validating stability study indicating method as per regulatory guidelines [ICH Guidelines].
[][]To set up specification of degradants or impurities and to identify structure and toxicity.
[][]To set propose shelf life the specific product without performing Realtime stability data.
[][]To avoid incompatibility of drug products and excipients.
[][]Determination of the process related degradation products or impurities.
[][]Provide supporting data to lab investigations/OOS [out-of-specification] analysis.
[][]To provide regulatory compliance documents during submitting of ANDA/NDA to FDA.

It is a useful tool to predict the stability of any Active Pharmaceutical Ingredient (API) or formulation product. It helps to know about the impurities developed during the storage of drug products in various environmental conditions.

Forced degradation is performed by applying artificial methods and a drug is degraded forcefully. It is also known as stress testing. To assume the stability condition of API [Active Pharmaceutical Ingredient] and formulated product Forced degradation study plays an important role. It also helps to identify the impurities generated during storage of drug products in different environment stage.

Why Forced degradation study carried out?

Its play a vital role to develop and validating of stability study signifying method. At the time of developing phase of a new drug product, force degradation study performs to determine the degradation pathways of drug products & drug substances. It is very important to determine impurities of the degradant product, Forced degradation study quantify the number of impurities present on the specific drug substance. It helps to determine the molecular chemistry. Forced degradation study assure the more stable product. Help to develop the degradation profile. Stability related problem can be solved through Forced degradation study. Forced degradation study also highlight the following point of view-

[][]Evaluation of drug products & drug substance in solution.
[][]Determination of structural transformation of drug product & drug substance.
[][]Determination of the concentration of the degradation products.
[][]To identify the non-relevant impurities in the existence of the desired product.
[][]Separation of the product related degradants derived from intact placebo & excipients.
[][]Describe the degradation pathways of the specific drug substance.
[][]To categorize the degradation products which generate spontaneously during storage & use of products.
[][]To generate product related variants & develop analytical methods Forced degradation studies are performed during accelerated and long-term studies.

During the Forced degradation study, the degradation products may or may not be generate but it will show the degradation pathway of the product. This process will help them develop the analytical method of the relevant product and stability indicating analytical procedure. If any degradation occurs during performing of Forced degradation study, the degradation product shall be evaluated if it significant or minute, to robust the developed formulation.

How Forced degradation Study Conducted?

This study of the drug products or substances is generally conducted on the solid and solution stages at the high temperature exceeding accelerated stability condition which is above 40°C. Various condition are consider here as oxidation, hydrolysis, photolysis, polymerization and thermolysis. In Solution hydrolysis condition are investigated in broader pH range and in solid stage high relative humidity taken under consideration.

Control exposure of molecular oxygen or addition of oxidizing agents such as peroxides is use during investigating Oxidation in solution.
Applying heat in solid state effects of thermolysis are usually assessed. Light with wavelengths in the 300-800 nm range are use in Photolysis investigation in solution or the solid state. In an oxygen atmosphere photooxidation can be investigated with light under oxygen atmosphere. Measuring the rate of degradation, Drug substance polymerization can be investigated at the various drug substance concentrations in solution.

List of Analytical Tools to perform Separation & Identification of degradant

A. Sophisticated Techniques

[][]Capillary Electrophoresis- Mass Spectrometry [CE-MS].
[][]Gas chromatography–mass spectrometry [GC-MS].
[][]Liquid chromatography–mass spectrometry [LC-MS].
[][]Liquid Chromatography- Nuclear magnetic Resonance [LC-NMR].
[][]Liquid chromatography-Fourier Transfer Infrared [LC-FTIR].

B. Conservative Techniques:

[][]Thin layer chromatography [TLC].
[][]Solid phase extraction [SPE].
[][]Accelerated solvent extraction [ASE].
[][]Low-pressure LC [LPLC].
[][]Supercritical fluid extraction [SFE].
[][]Mass Spectrometry [MS].
[][]Nuclear Magnetic Resonance [NMR].
[][]High Performance Liquid Chromatography [HPLC].

Extend of degradation

For validation of a chromatographic purity assay, degradation level of 10-15% is adequate to perform the activities. Forced degradation studies are not considered part of the formal stability program though forced degradation studies are a regulatory requirement & scientific necessity during development of a specific product. For conducting studies at the various phases of development the guidance gives various recommendations.

Selection of Forced Degradation Condition

In common industry practice, forced degradation is generally performed in different stress conditions, i.e., thermal, acid, alkali, peroxide, and UV, along with a control sample which also comply with ICH guidelines. There no specific range or rate of degradation in current industry practice but 5 to 30 percent degradation shall be taken into consideration and this can be achievable on any one of the above stress conditions.

Through stress testing, the aim of the degradation to be achieved to implement the control room temperature for the stability conditions. The conditions or concentrations of reagent shall be optimized if higher or lower degradations are observed.

During the degradation study Mass balance shall be demonstrated & it shall be around 100%, taking into attention margins of analytical errors. During mass balance evaluations, all the degradants /impurities must be calculated.

Any batch which is not be the part of regulatory submission can be used for the forced degradation study. For multiple strengths of the same placebos and different amounts, the highest ratio of placebo vs. API [Active pharmaceutical ingredient] shall be use.

Forced degradation of all the strengths shall demonstrate if placebos are different. Placebo & API [Active Pharmaceutical Ingredient] must be demonstrated to identify actual degradation pathways during the drug product force degradation study. All the placebos shall be considered for force degradation study if placebos are different for different strengths of drug product.

Various degradation conditions are depicted on the following table which is accepted by the regulatory authority [FDA] at the time of DMF/ANDA/NDA submission-

Degradation TypeReagent ConcentrationConditions to be appliedTimeRemarks
Acid5N HCL80deg.C1 HourConcentration, condition and time can change to optimize degradation
Alkali5N NaOH80deg.C1 HourDo
Peroxide10% H2O280deg.C1 HourDo
Heat/Thermal80deg.C80deg.C1 HourDo
UVExpose under UV light at 254nm wavelengthAmbient Temperature24 HoursTime can change to optimize degradation
ControlN/AN/AN/AN/A

Force Degradation shall be performed in solid or solution form though it is recommended that Force Degradation shall be executed in solution form using the mobile phase/diluent to get a homogeneous effect with better result. Force Degradation studies shall be started with harsh conditions (i.e., high temperature with high concentration of reagent) to shorten time of study.

Milder conditions shall be applied by reducing concentration of reagent with lowering temperature, etc. when degradation found 30% or above. Based on the initial degradation outcome, Degradation conditions can be optimized to achieve a target range.

To extend shelf life of chromatographic column, pH shall be adjusted about 7.0 for acid & alkali degradation. Different reagents & conditions shall be applied, e.g., Zn, H2SO4, etc. If degradation did not find in any of above conditions. A few numbers of molecule designated rock stable molecules as these molecules didn’t degrade any of the above stress condition. During a stability study This kind of molecule will not engender any additional impurities/degradant peaks.

If drug substance or product shows stability for two years at 30 ±2⁰C & 65 ±5% RH & Six months at 40 ±2⁰C & 75 ±5%RH, then the drug substance or product declared stable.
Concentration of the drug that is being tested for the degradation is a great point. For the degradation study 1 mg/ml of drug concentration is recommended though some degradation studies are done at concentration of drug in the final product. Main cause for this type of study is that precise amount of the degradation can be found in final product & their impact can be scrutinized.

Factors Affecting Forced Degradation Studies

Hydrolytic Degradation:

The reaction of chemical with water at different pH values occur in Hydrolysis degradation. In this degradation drug react with water in acidic & basic conditions. According to the stability of the drug substance concentration of the acid or base is selected where pH is 0.1 to 1.0 M HCl [Hydrochloric Acid] or H2SO4[Sulfuric Acid].

HCl & H2SO4 is used to maintain acidic conditions and 0.1 to 1.0M NaOH [Sodium hydroxide] or KOH [Potassium Hydroxide] used to generate basic conditions. Some materials are not readily dissolve/soluble in water freely; in that case other solvent are use to dissolve the water insoluble materials. Solvent shall be selected carefully so that it can’t degrade the selected drug substance.

Descriptive termPart of the solvent require per part of solute
Very solubleLess than 1
Free SolubleFrom 1 to 10
SolubleFrom 10 to 30
Sparingly solubleFrom 30 to 100
Slightly solubleFrom 100 to 1000
Very Slightly solubleFrom 1000 to 10,000
Practically insoluble10,000 and over

Reference: British Pharmacopoeia [BP]

Generally Chemical degradation shall be conduct in room temperature but if no sign of Chemical degradation occur at room temperature then room temperature shall be increase up to 50-60 ⁰C. A seven days timeframe shall be selected to perform the study. To prevent further degradation, Chemical degradation should be terminated using acid, base or buffer solution. Chemical analysis shall be done as soon as possible after completion of the test.

Oxidation Degradation:

in the forced degradation study, H2O2 (Hydrogen peroxide) is a widely used oxidizing agent. Hydrogen peroxide at 0.1% to3.0% solution is used at room temperature for 7 days is the suitable range to perform the activities. When more then20% degradation occur for a certain product, it can be considered abnormal cases.

Photolytic Degradation:

To determine the effect of light on the product during storage in the market Photostability testing of any drug take into consideration. light conditions shall be described during photostability. light source shall be cool white fluorescent lamp & wavelength of light shall be 200-800 nm (UV+ visible) which is also comply ICH guideline. The and the light intensity shall be not less than 200 watt-hours per sq meter and exposure time shall be not less than 1.2 million lux hours. To monitor the condition, a calibrated lux meter shall be use in place.

Result of forced degradation studies

[][]Forced degradation studies help to determine_
[][]Likely/Probable degradants
[][]Degradation paths
[][]Inherent stability of the drug molecule
[][]Validated stability indicating analytical method

When forced degradation studies to be performed?

This is the best practice to perform forced degradation studies at the time of development of new drug substance and new drug product. FDA prefer to perform it at phase III of the regulatory submission which is the best time to do the same. To establish the regular stability study, forced degradation studies can be prerequisite. This study can be done in different pH solution in the presence of light & Oxygen with high temperature & Humidity Level.

Generally, degradation study performs on single batch. There are two types of timeframes are use to perform stability study which Long Term [12 Months] & Short Term [6 Months]. 6 Months are performed at accelerated condition. Moreover, Intermediate Stability Study performed in a condition lesser than accelerated condition.

Force degradation studies are performed at pre-clinical phase or phase I of clinical trial so that sufficient time provides to identifying structure elucidation, degradation products. If forced degradation studies are performed properly, manufacturing process of the new product can be developed properly and stability-indicating analytical procedures can be select more effectively.

What is the regulatory obligation regarding Force Degradation Studies?

Following ICH [International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use] Guidelines has been depicted regarding forced degradation studies but it covers only marketing applications for new products but not for during clinical development.

[][]ICH Q1A: Stability Testing of New Drug Substances and Products.
[][]ICH Q1B: Photo stability Testing of New Drug Substances and Products.
[][]ICH Q2B: Validation of Analytical Procedures: Methodology.

What actually says this guideline?

[][]ICH Q1A (Stress testing): Stability Testing of New Drug Substances and Products.

It implies for the performing of forced degradation studies for drug substances and drug products. The recommended condition is that the result shall be observe temperature above accelerated condition [Temperature>500C) and Humidity [75% relative humidity] including oxidation and photolysis. pH range may be wide for the testing of solution or suspension.

[][]ICH Q1B: Photo stability Testing of New Drug Substances and Products.

It implies the photo stability of drug substances and drug products. Section II and Section III describe the forced degradation conditions condition for drug substance and drug product. Exposure levels are not defined in Forced degradation studies. Photo stability testing can be performed both in Solid or in solution/suspension. Stability indicating method is developed based on this sample result. Some non-experiential degradation products may be formed during stability studies which may not be taken under consideration.

[][]ICH Q2B: Validation of Analytical Procedures: Methodology.

Provide guideline regarding analytical meth validation. Gives guidance to validate the analytical methodology. To demonstrate specificity, in section B1.2.2 (impurities not available) there is a recommendation to utilize samples from the forced degradation studies.

Verdict

to develop degradation pathways, Forced degradation studies are the prominent way & Forced degradation studies are the prominent way to develop degradation pathways and to detect degradation products of API [Active pharmaceutical Ingredients], further it simplifies elucidation of degradants structure. Forced degradation studies also simplify the chemical & physical stability analysis of drug substances & drug products. To develop manufacturing conditions, storage conditions & determine expiry date of a new drug formulation Forced degradation studies is considered as key studies.

Forced Degradation Study or Stress Testing Procedure Read More »

Comparative Dissolution Study procedure

Comparative Dissolution, General Overview

[][]Comparative Dissolution, Oral dosage form like Table & Capsule are more popular than IV/IM(Intravenous/Intramuscular) Injection formulation. From the very beginning, people are most familiar with oral solid dosage form (Tablet, Capsule, Powder etc.) and becoming more popular till today as no special technique or device is not require to administer these products and associated pain is not involved here.

[][]In the period of time its are considered as the most effective and efficient method to treat the patient. This orally taken drugs are dissolved in GI (Gastro Intestinal) fluid and then bioavailable at the systemic circulation as it absorbed here. To measure the bioavailability of a certain drugs (in vivo Analysis, vivo is Latin for “within the living, test perform in living organism) is not accurately possible due to its complex nature.

[][]For this reason, in vitro (vitro is Latin for “within the glass, test perform outside the living organism) methods are followed to measure the dissolution rate of a certain drugs. This method is officially recognized by certain regulatory authority and it(in vitro study) considered most convenient way to develop new formulation of oral solid dosage form.

Comparative Dissolution Consideration

[][]Dissolution method is the best option for the lower strength drug where different strength is proportionally formulated to acquire the biowaiver of certain formulated drug. For a certain product which higher strength bioequivalence study has been carried out and found proportional to the concentration then biowaiver is conceivable to the lower strength.

Dissolution test are considering the most quality control tool for the commercial batch to batch product to monitor its consistency over a certain period of time. It also provides significant information during post approval changes of the certain product as changes made in formulation, manufacturing process and different scale up procedure.

[][]The most physiological factor is considered as the dissolution and solubility of the API and its permeability through the membrane of the GI[Gastro Intestinal] tract. As this measurement is so prone to error due to its complex nature then in vitro study consider the most convenient and reliable procedure to achieve the required target. During development of a certain solid dosage form, dissolution is considered as the best option to determine its quality parameter which have the great impact on the bioavailability of the formulated product.

Comparative Dissolution, Waiver of in vivo bioavailability

[][]BCS system applied in this case so that waiver for in vivo analysis can be assured. BCS [Biopharmaceutical Classification System] is a system which measure the permeability and solubility of drugs in a certain prescribed condition.

[][]The actual aim of BCS is to aid the post approval changes and arranging approval activities based on in vitro data studies.

This system has been optimized based on the oral solid dosage unit as most of the market products are available at oral dosage form [More than 50% total market share, US$23.4 Bn in 2021, US$24.7 Bn in 2022 as estimated, growth rate 5.9%).

[][]Waivers[ means giving permission to skip in vivo bioequivalence study] is actually reserved for those products that meet the specific requirements of solubility & permeability & most of the cases rapid dissolve in body fluid.

[][]Using the BCS, appropriate formulation study shall be developed such as Type II drugs designed as Permeable but insoluble, this class is not the actual right candidate for development of a new moiety.

[][]So, solubility shall be developed to acquire the right dissolution profile. Based on the solubility and permeability BCS has classified the four categories of the product as depicted below

Dosage form challenge

[][]Comparison has been drawn from old drug to new drug formulation, where older drugs compare to the current products are more prone to solubilities. Class II compound has been remarkably increased as 30% to 60% where class I compound has down to 40% to 20% where low solubility has the main cause to encounter the issue.

 A oral solid dosage form is the preferred option but all time this can’t possible the suspension or solution is continued to prove its existence  

[][]Generally a highly soluble active substance and rapidly dissolve dosage form provide better bioavailability and in this case biowaiver can be waived for bioequivalence studies base on its dissolution profile.

[][]If a active substance found low solubility but high permeability then the rate limiting steps of absorption may be consider as dissolution. Most of the cases dissolution profile control the more than one of excipients or special design matrix compounds. So Test condition may be consider as various time frame (10, 15, 20,30, 45 & 6 minutes).

[][]Drugs that are poor soluble in water then various time frame are considered and accepted timeframe is set for dissolution profile. Here USP Type 4 apparatus to be used to develop such type dissolution profile. Most of the time, monograph for combination product is not available at BP or USP the individual monograph shall be used to set the dissolution profile.

Selection of Dissolution Media

Selection of the dissolution media is the vital point to achieve the goal. pH of the media as the key role as all of the dosage form goes to GI[Gastro Intestinal] tract so pH shall be simulate with the GI Tract environment. pH shall be 1.2 to 6.8 which is the physiologic pH range of the body.

ZonepH
[A]Pre-prandial
Stomach
1.8(1~3)
Duodenum6.0(4~7)
Upper Jejunum 6.5(5.5~7)
Lower Jejunum 6.8(6~7.2)
Upper Ileum7.2(6.5~7.5)
Lower Ileum7.5(7~8)
Proximal Colon(5.5 ~6.5)
[B]Post-prandial
Stomach
4.0(3~6)
Duodenum5.0(4~7)
Upper Jejunum 5.5(5.5~7)
Lower Jejunum 6.5(6~7.2)
Upper Ileum7.2(6.5~7.5)
Lower Ileum7.5(7~8)
Proximal Colon(5.5 ~6.5)

Dissolution Statistics

Different cases obtaining after multipoint dissolution which is calculative as follows:
[][]If Test Product and Reference Product both shows dissolution rate more than 85% within first 15 minutes then no calculation is required, they are considered as similar. If it didn’t achieved then seek for next step.
[][]Seek for f2 value[ f2, similarity factor] if f2>50% then it consider similar then in vivo study is not required.
[][]Difference Factor [f1] is the percentage (%) difference between the two curve at the each time period and also measure the relative error between two curve.

How it works

To determine the difference and similarity factor(f2) following pont shall be noted:
[][]Use the Two different products for study, from each product collect 12 unit [12 unit from Test Product & 12 Unit from Reference Product].

[][]Three time point shall be considered[Exclude Point Zero], only one measurement after 85% shall be measured.
[][]Produced curve shall be similar, f2 values shall be close to 100. Most of the time f2 value more than 50 denote similarity of the two curves as well as equivalence of the two products.

 If Three/Four Time points come to the test then following points shall be considered. 

[][]The measurement for the Test Product and the Reference product shall be same. Dissolution Time point shall be same for the both product (10,15,20,30,45,60 minutes etc.). Products which tend to faster dissolution (85% dissolve within 30 minutes) then time frame shall be consider as 10, 15, 20, 30 minutes.
[][]Only One measurement shall be consider after completion of 85% dissolution of both sample and reference products.

System Requirements to Perform Comparative Dissolution

Dissolution Activities shall be continued on USP Type I Dissolution apparatus at 100 RPM or USP Type II Apparatus at 50 RPM using 900 ml of different dissolution media mentioned below.
[][]Media Use in Comparative Dissolution
[][]Acid Media: 0.1N HCL or Simulated Gastric Fluid USP without Enzyme
[][]Acetate Buffer pH 4.5
[][]Phosphate Buffer pH 6.8 or Simulated Intestinal Fluid without Enzyme
[][]If both the Test Product and Reference product shows more than 85% dissolution within first 15 minutes then no calculation required. If not meets the above requirements then calculate f2 Value.
[][]If found f2>50, then the profile considered as similar and in vivo study is not required. Minimum 12 unit of each shall be consider for comparative dissolution.

Comparative Dissolution Study procedure Read More »

Performance Qualification of WFI Phase 3

Performance Qualification of WFI,  Purpose

Performance Qualification of WFI, To authenticate and document that the performance of the WFI Generation and Distribution System installed at the WFI & PS plant room (roof top) of Cephalosporin Block of XX Pharmaceuticals Limited is satisfactory in all critical features related to the operational requirements during Phase – 3 study.

Performance Qualification of WFI, Scope

This protocol will be applicable for performing Phase – 3 validation study to verify that the Water For Injection generation and distribution system installed at WFI & PS plant room (roof top) of Cephalosporin Block of XX Pharmaceuticals Limited consistently produce desired quality of Water For Injection over the study period of 01 year.

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Water For Injection Performance Qualification of Phase 2 Study

Responsibilities

[][]Preparation of the protocol
Validation (Engineering) Department.
[][]Executing the protocol
Validation team along with respective departmental person and engineers or other suitably qualified staff allocated from the site or contracted specialists as appropriate.
[][]Chemical test
Quality Control Department
[][]Microbiological Test
Microbiology Department
[][]Data documentation and preparation of the report
Validation (Engineering) department.
[][]Verifying the report
Concerned departmental person.
[][]Providing documentation on the equipment
Related departments i.e. Engineering, Quality Control, Microbiology, Validation.

System And Process Description

System Information

=>Manufacturer : Watertown
=>Capacity : 750 ltr/hr
=>Model : MS750/4T
=>Manufacturer no. : MS1132

The System under test

[][]The Water For Injection system is designed to generate and distribute the desired quantity & quality of Water For Injection to various user points.

Generation system of Water For Injection

[][]The WFI production process consists of purified water evaporation followed by pure steam separation and condensation through four multi-effect water still columns.

[][]Purified water from the storage tank is pressurized through a feed water pump and pre heated in the four pre heaters.

[][]Then, the purified water becomes pure steam in the four multi effect columns by exchanging heat with the plant steam.

[][]There are two condensers in the system. The first condenser is used to cool the pure steam by exchanging heat with the incoming feed water (PW).

[][]Finally, the WFI is produced in second condenser by exchanging heat with the chilled water which is then supplied to the WFI storage tank.

Distribution system of Water For Injection

[][]The WFI is stored in a WFI storage tank of 2000 ltr capacity and is distributed to the user points and circulated within a loop by a high pressure pump.

[][]There is a heat exchanger in the distribution section to sterilize the whole distribution system.

Tests To Be Performed And Sampling Plan

[][]A comprehensive validation study plan has been established where a weekly sampling plan has been developed for Phase-3 study of WFI system for 01 year concerning weekly testing of all main sampling points.

[][]The sampling will be taken one day a week on working days. The acceptance criterion of the tests are stated in the table.

Main Sampling Points

The sampling points of WFI are listed below:

Sl. No.Sample IDUser point LocationTestsMicrobial Count/
Chemical Tests
01WSP-1Final WFI after condenser – 2 (before WFI storage tank).Next TableWeekly
02WSP-2At supply of WFI Distribution LoopNext TableWeekly
03WSP-3At return of WFI Distribution LoopNext TableWeekly
04WUSP – 01WFI Distribution LoopNext TableWeekly
05WUSP – 02WFI Distribution LoopNext TableWeekly
06WUSP – 03WFI Distribution LoopNext TableWeekly
07WUSP – 04Cold User pointNext TableWeekly
Tests and Acceptance Criteria for Water for Injection

Sl. No.TestsFrequencyAcceptance CriteriaAlert LimitAction Limit
01Appearance Daily from each sampling point.Clear, colorless and odorless liquid.N/AN/A
02Conductivity Daily from each sampling point.Not more than 1.1 µS/cm at 20⁰C or
Not more than 1.3 µS/cm at 25⁰C
0.7 µS/cm0.9 µS/cm
03Total Organic CarbonDaily from each sampling point.Not more than 500 ppb106.4 ppb135.8 ppb
04Total Viable Microbial CountDaily from each sampling point.Not more than 10 CFU/100 mL5 CFU/100 mL8 CFU/100 mL
05E.coliDaily from each sampling point.Must be AbsentN/AN/A
06Staphylococcus aureusDaily from each sampling point.Must be AbsentN/AN/A
07Pseudomonas aeruginosaDaily from each sampling point.Must be AbsentN/AN/A
08Salmonella sppDaily from each sampling point.Must be AbsentN/AN/A
09Bacterial EndotoxinsDaily from each sampling point.Less than 0.25 EU /mLN/AN/A

[][]Performance Qualification shall be considered acceptable when all the conditions above have been met.
[][]Any deviation from the acceptance criteria of the specific check point shall be reported and decision should be taken for the rejection, replacement or rectification of the equipment/component/system.

Conclusion

[][]The results of the Phase – 3 study, as per the qualification protocol are recorded & analyzed.

[][]The observed parameters/tests and subsequent analytical results show that the Water For Injection Generation and Distribution System Complies/Does Not Comply as per the predetermined acceptance criteria.
[][]Hence, the Water For Injection Generation and Distribution is / is not qualifying the Phase – 3 study of Performance Qualification and the System can be / cannot be used for production purpose and continued to regular monitoring by Quality Control and Microbiology department.

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Performance Qualification Protocol of WFI Phase 3

Performance Qualification of WFI Phase 3 Read More »

Water For Injection Performance Qualification in Phase 2 Study

Water For Injection Performance Qualification, Purpose

Water For Injection Performance Qualification, To validate and archive that the exhibition of the WFI Generation and Distribution System introduced at the WFI and PS plant room (rooftop top) of Cephalosporin Block of XX Pharmaceuticals Limited (XPL) is agreeable in all basic elements connected with the functional necessities during Phase – 2 Study.

Water For Injection Performance Qualification, Scope

This Protocol will be relevant for performing Phase – 2 study to check that the Water For Injection generation and conveyance framework introduced at WFI and PS plant room (rooftop top) of Cephalosporin Block of XX Pharmaceuticals Limited, reliably produce wanted nature of Water For Injection over the review time of 20 continuous working days.

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Water For Injection Performance Qualification Phase I Study

Responsibilities

[][]Preparation of the protocol
Validation (Engineering) Department.
[][]Executing the protocol
Validation team along with respective departmental person and engineers or other suitably qualified staff allocated from the site or contracted specialists as appropriate.
[][]Chemical test
Quality Control Department
[][]Microbiological Test
Microbiology Department
[][]Data documentation and preparation of the report
Validation (Engineering) department.
[][]Verifying the report
Concerned departmental person.
[][]Providing documentation on the equipment
Related departments i.e. Engineering, Quality Control, Microbiology, Validation.

System & Processing

System Information
Manufacturer : Watertown
Capacity : 750 ltr/hr
Model : MS750/4T
Manufacturer no. : MS1132

The System under test

The Water For Injection system is designed to generate and distribute the desired quantity & quality of Water For Injection to various user points.

Generation system of Water For Injection

[][]The WFI manufacturing process consists of purified water evaporation followed by pure steam separation & condensation through four multi-effect water still columns.

[][]Purified water from the storage tank is pressurized through feed water pump & pre heated in the four pre-heaters.

[][]Then, the purified water becomes pure steam in the four multi effect columns by exchanging heat with the plant steam.

[][]There are two condensers in the system. The first condenser is used to cool the pure steam by exchanging heat with the incoming feed water (PW).

[][]Finally, the WFI is produced in second condenser by exchanging heat with the chilled water which is then supplied to the WFI storage tank.

Distribution system of Water For Injection

The WFI is stored in a WFI storage tank of 2000 L capacity and is distributed to the user points and circulated within a loop by a high pressure pump. There is a heat exchanger in the distribution section to sterilize the whole distribution system.

Test to be perform & Sampling

[][]A comprehensive validation study plan has been established where a daily sampling plan has been developed for Phase-2 study of WFI system for 20 consecutive working days concerning daily testing of all main sampling points.The acceptance criterion of the tests are stated in the table of section.

Main Sampling Points

The sampling points of WFI are listed below:

Sl. No.Sample IDRoom NameTestsMicrobial Count/
Chemical Tests
01WSP-1WFI and Pure Steam plant roomBelow TableDaily
02WSP-2WFI and Pure Steam plant roomBelow TableDaily
03WSP-3WFI and Pure Steam plant roomBelow TableDaily
04WUSP – 01Vial Washing & SterilizationBelow TableDaily
05WUSP – 02Laundry & WashBelow TableDaily
06WUSP – 03Wash BayBelow TableDaily
07WUSP – 04Laundry & WashBelow TableDaily

[][]Performance Qualification shall be considered acceptable when all the conditions above have been met.
[][]Any deviation from the acceptance criteria of the specific check point shall be reported and decision should be taken for the rejection, replacement or rectification of the equipment/component/system.

Tests and Acceptance Criteria for Water for Injection (Based on Current USP 37)

Sl. No.TestsFrequencyAcceptance CriteriaAlert Limit
01Appearance Daily from each sampling point.Clear, colorless and odorless liquid.Not Available
02Conductivity Daily from each sampling point.Not more than 1.1 µS/cm at 20⁰C or
Not more than 1.3 µS/cm at 25⁰C
Not Available
03Total Organic CarbonDaily from each sampling point.Not more than 500 ppbNot Available
04Total Viable Microbial CountDaily from each sampling point.Not more than 10 CFU/100 mLNot Available
05E.coliDaily from each sampling point.Must be AbsentNot Available
06Staphylococcus aureusDaily from each sampling point.Must be AbsentNot Available
07Pseudomonas aeruginosaDaily from each sampling point.Must be AbsentNot Available
08Salmonella sppDaily from each sampling point.Must be AbsentNot Available
09Bacterial EndotoxinsDaily from each sampling point.Less than 0.25 EU /mLNot Available
Reference Documents

Sl. No.Title of the DocumentDocument No.
01WHO Good Manufacturing Practices: water for pharmaceutical use.WHO Technical Report Series, No. 929, 2005
02WHO Expert Committee on Specifications for Pharmaceutical Preparations.WHO Technical Report Series No. 970, 2012
03United States Pharmacopeia 37Not Available

Conclusion

[][]The results of the Phase – 2 study, as per the qualification protocol are recorded & analyzed. The observed parameters/tests and subsequent analytical results show that the Water For Injection Generation and Distribution System Complies/Does Not Comply as per the predetermined acceptance criteria.

[][]Hence, the Water For Injection Generation and Distribution is / is not qualifying the Phase – 2 study of Performance Qualification and the System can be / cannot be used for production purpose and continued to Phase – 3 study.

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Water For Injection Performance Qualification Phase 2 Study

Water For Injection Performance Qualification in Phase 2 Study Read More »

WFI Performance Qualification, Phase-I Study

WFI Performance Qualification, Purpose

WFI Performance Qualification, To authenticate and document that the performance of the WFI Generation and Distribution System installed at the WFI & PS plant room of Cephalosporin Block of XX Pharmaceuticals Limited (XPL) is satisfactory in all critical features related to the operational requirements during Phase – 1 (Investigational Phase) study.

WFI Performance Qualification, Scope

This protocol will be applicable for performing Phase -1 validation study to verify that the Water For Injection generation and distribution system installed at the utility area (roof top) of Cephalosporin Block of XX Pharmaceuticals Limited,  consistently produce desired quality of Water For Injection over the study period of 20 consecutive working days.

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Pure Steam System Performance Qualification

Responsibilities

[][]Preparation of the protocol
=>Validation (Engineering) Department.
[][]Executing the protocol
=>Validation team along with respective departmental person and engineers or other suitably qualified staff allocated from the site or contracted specialists as appropriate.
[][]Chemical Test
=>Quality Control Department
[][]Microbiological Test
=>Microbiology Department
[][]Data documentation and preparation of the report
=>Validation (Engineering) department.
[][]Verifying of the report
=>Concerned departmental person.
[][]Providing documentation on the equipment
=>Related departments i.e. Engineering, Quality Control, Microbiology, Validation.

System And Process Description

=>System Information

=>Manufacturer : Watertown
=>Capacity : 750 ltr/hr
=>Model : MS750/4T
=>Manufacturer no. : MS1132

The System under test

The Water For Injection system is designed to generate and distribute the desired quantity & quality of Water For Injection to various user points.

Generation system of Water For Injection

[][]The WFI production process consists of purified water evaporation followed by pure steam separation and condensation through four multi-effect water still columns.

[][]Purified water from the storage tank is pressurized through a feed water pump and pre heated in the four pre heaters.

[][]Then, the purified water becomes pure steam in the four multi effect columns by exchanging heat with the plant steam.

[][]There are two condensers in the system. The first condenser is used to cool the pure steam by exchanging heat with the incoming feed water (PW).

[][]Finally, the WFI is produced in second condenser by exchanging heat with the chilled water which is then supplied to the WFI storage tank.

Distribution system of Water For Injection

[][]The WFI is stored in a WFI storage tank of 2000 ltr capacity and is distributed to the user points and circulated within a loop by a high pressure pump.

[][]There is a heat exchanger in the distribution section to sterilize the whole distribution system.

Tests To Be Performed And Sampling Plan

[][]A complete validation study plan has been designed in accordance with the Validation Master Plan. In addition, a daily sampling plan has been also developed for Phase-1 study of WFI system for 20 consecutive working days concerning daily testing of all main sampling points .

[][]The acceptance criterion of each test is stated in the next table.
[][]Alert and action limits will be calculated using following formula after getting the test results and data:
=>Alert limit = Average value + 2σ
=>Action limit = Average value + 3σ

Main Sampling Points

The sampling points of WFI are listed below:

Sl. No.Sample IDRoom NumberUser point LocationTestsMicrobial Count & Chemical Tests
01WSP-1SRT001Final WFI after condenser – 2 (before WFI storage tank).Mention on next TableDaily
02WSP-2SRT001At supply of WFI Distribution LoopMention on next TableDaily
03WSP-3SRT001At return of WFI Distribution LoopMention on next TableDaily
04WUSP – 01SPR054WFI Distribution LoopMention on next TableDaily
05WUSP – 02SPR040WFI Distribution LoopMention on next TableDaily
06WUSP – 03SMB010WFI Distribution LoopMention on next TableDaily
07WUSP – 04SPR040Cold User pointMention on next TableDaily

[][]Performance Qualification shall be considered acceptable when all the conditions above have been met.
[][]Any deviation from the acceptance criteria of the specific check point shall be reported and decision should be taken for the rejection, replacement or rectification of the equipment/component/system.

Tests And Acceptance Criteria For Water For Injection

Sl. No.TestsFrequencyAcceptance CriteriaAlert Limit
1Appearance DailyClear, colorless and odorless liquid.N/A
2Conductivity DailyNot more than 1.1 µS/cm at 20⁰C or
Not more than 1.3 µS/cm at 25⁰C
N/A
3Total Organic CarbonDailyNot more than 500 ppbN/A
4Total Viable Microbial CountDailyNot more than 10 CFU/100 mLN/A
5E.coliDailyMust be AbsentN/A
6Staphylococcus aureusDailyMust be AbsentN/A
7Pseudomonas aeruginosaDailyMust be AbsentN/A
8Salmonella sppDailyMust be AbsentN/A
9Bacterial EndotoxinsDailyLess than 0.25 EU /mLN/A
Test Records

All the test reports of chemical and microbiological tests are attached with the report as attachment.

Reference Documents

Sl. No.Title of the DocumentDocument No.
01WHO Good Manufacturing Practices: water for pharmaceutical use.WHO Technical Report Series, No. 929, 2005
02WHO Expert Committee on Specifications for Pharmaceutical Preparations.WHO Technical Report Series No. 970, 2012
03United States Pharmacopeia N/A

Download Here

WFI Performance Qualification, Phase-I Study Protocol

WFI Performance Qualification, Phase-I Study Read More »

Vial Washing Machine Performance Qualification

Vial Washing Machine Performance Qualification, Purpose

Vial Washing Machine Performance Qualification, To authenticate and document that the performance of the Vial Washing Machine of Sterile Production area (Cephalosporin Block) of XX Pharmaceutical Limited (XPL) is satisfactory in all critical aspects related to the operational requirements during washing of vials. This protocol describes the performance qualification procedures of the Vial Washing Machine manufactured by Tofflon, China.

Vial Washing Machine Performance Qualification, Scope

This Performance Qualification (PQ) is to be performed against agreed acceptance criteria on 7.5 ml, 15 ml and 30 ml vials which will be washed by the Vial Washing Machine installed in the Sterile Production area of Cephalosporin Block of XX Pharmaceutical Limited.

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Vial Filling Machine Performance Qualification

Responsibilities

[][]Preparation of the protocol

Validation (Engineering) Department with the assistance of Microbiology, Engineering and Maintenance department.
Executing the protocol

[][]Validation (Engineering) Department along with respective departmental person and engineers or other suitably qualified staff allocated from the site or contracted specialists as appropriate.
[][]Microbiological Challenge Test

Microbiology department
[][]Chemical Tests

Quality Control Department
[][]Data documentation and preparation of the report

Validation (Engineering) department.
[][]Verifying of the report

Concerned departmental person.
[][]Providing documentation on the equipment

Related departments i.e. Engineering, Production, Microbiology, Validation or other appropriate departments and manufacturer.

System And Process Description

[][]Equipment Information
=>Manufacturer : Tofflon, China
=>Capacity : 6000 – 9000 vials/hr.
=>Model : QCLX60
=>Serial no. : YF2014-078A

[][]The Equipment under test
The vials from the box are placed on the infeed conveyor belt and are showered by recycled WFI at first. Then, these are cleaned in the ultrasonic bath and transferred to the grippers. The infeed rotary screw transports the vials from the housing to the grippers. There are total twenty stations where 60 vials are washed using following sequence:
=>Internal blowing with compressed air.
=>Internal and external spraying with recycled WFI at ambient temperature.
=>Internal blowing with sterile compressed air.
=>Internal and external spraying with fresh WFI.
=>Internal blowing with compressed air.
=>Internal and external blowing with sterile compressed air.
=>The supply lines are equipped by filter with the following retention grade: compressed air 0.22 µm, recycled WFI 10 µm and 5 µm, and fresh WFI 0.22 µm.

Checking Parameters

The PQ of Vial Washing Machine will be carried out to evaluate the following conditions to confirm the sterilization conditions:
i) Riboflavin Test.
ii) Particulate Matter Test.
iii) Bacterial Endotoxin Test.
iv) Washing Efficiency Test.

Study Procedure:

[][]Riboflavin Test: Take the Vials and do the decartoning and check for any Physical deformity or damage. A 0.2 gm riboflavin sodium phosphate per liter of purified water solution is prepared.

[][]Take 60 vials, mark with the permanent marker and spray the interior and exterior of the vials with the solution so that every vial is properly moistened with the solution and keep for overnight.

[][]Next morning dry the vials in oven for 2 hrs at 150 °C. After that, rinse the vials with phosphate buffer solution of pH 7.0 and measure the absorption in a UV-VIS spectrophotometer using 1 cm cell at a wavelength 266 nm by setting the limit between 230 nm and 350 nm.

[][]Use phosphate buffer solution of pH 7.0 as blank. Record the spectrum. Place the marked 60 Vials with these and run the machine as per SOP and collect these vials after washing.

[][]Take these vials again; repeat the procedure in order to confirm the presence of any riboflavin on vials.
The test will be repeated at minimum and maximum speed of washing machine for each of three vials’ sizes 7.5ml, 15ml and 30ml.

[][]The results of above test will be attached with the report and will be accepted if met the acceptance criteria.

Acceptance Criteria:

Riboflavin content must be absent in all the spiked vials after washing.

Particulate Matter Test:

[][]10 gm of charcoal is dissolved in 1000 gm WFI to make 1% charcoal slurry.

[][]Approximately 0.1 ml of charcoal slurry is used to spike each of 60 vials by swirling the vial to coat the inner surface and evaporate the solution to dryness at room temperature.

[][]Spiked test vials with charcoal are marked in series with permanent marker on the outer surface.

[][]Take the particle count of the vials before washing. After that, load these vials on the tray and run the machine according to the SOP No.____________________.

[][]The test must be performed at minimum and maximum speed of washing machine for each of three vials’ sizes 7.5ml, 15ml and 30ml.
[][]The results of above test will be attached with the report and will be accepted if met the acceptance criteria.

Acceptance Criteria:

Vials should be free from foreign particle & fibers on visual inspection. 10µ particles should not be more than 25 and 25µ particles should not be more than 3.

Bacterial Endotoxin Test:

[][]Add 100 endotoxin units into 10 vials and allow them to dry at 45 – 50°C. Mark these spiked vials with permanent marker on outer surface.

[][]Separate 10 vials are used for each of above tests. About 3000 vials of selected size are loaded on the feed belt and vial washing machine is operated as per the standard operating procedure.

[][]Place the spiked vials marked with permanent marker in between the other vials while machine is running. Collect the marked vials after washing and store properly to avoid the other contamination from environment.

[][]These spiked vials are analyzed separately. It should be done three times for each vial size. The test must be performed at minimum and maximum speed of washing machine for each of three vials’ sizes 7.5ml, 15ml and 30ml.
[][]The results of above test will be attached with the report and will be accepted if met the acceptance criteria.

Acceptance Criteria:

Vials should comply endotoxin limit within 0.25 EU/vial.

[][]Following parameter also maintained as per protocol

=>Deviation And Failure Investigation Summary

=>Documentation Requirements

=>Report Summary

Conclusion

The performance qualification Complies / Does Not Comply with the acceptance criteria taken above and the results obtained are attached with the protocol.
The Vial Washing Machine of Sterile Production area of Cephalosporin Block is / is not qualifying the Performance Qualification test as per this Protocol. Therefore, the Vial Washing Machine can be / cannot be used for production operation.

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Vial washing Machine Performance Qualification Protocol

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Laminar Air Flow Performance Qualification

Laminar Air Flow Performance Qualification, Purpose

Laminar Air Flow Performance Qualification, To authenticate and document that the performance of the Laminar Air Flow unit of Sterile Production area of Cephalosporin Block of XX Pharmaceuticals Limited (XPL) meets the user’s requirements of XPL Pharmaceutical Limited.

Laminar Air Flow Performance Qualification, Scope

This Performance Qualification (PQ) is to be performed against agreed acceptance criteria on the Laminar Air Flow Unit, which has been installed above the turn table after Depyrogenation Tunnel in Room of Sterile Production floor of Cephalosporin Block.

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Responsibilities

  • Preparation of the protocol
    -Engineering Department with the assistance of Validation team.
  • Executing the protocol
    -Validation (Engineering) Department along with respective departmental person and engineers or other suitably qualified staff allocated from the site or contracted specialists as appropriate.
  • Microbiological Test
    -Microbiology department
  • Data documentation and preparation of the report
    -Validation (Engineering) department.
  • Verifying of the report
    -Concerned departmental person.
  • Providing documentation on the equipment
    -Related departments i.e. Engineering, Production, Microbiology, Validation or other appropriate departments and manufacturer.

Performance testing

  • Air borne particle count test
    =>Purpose: the purpose of this test is to ensure that the air borne particles are under the acceptance limit.
    =>Test method: carry out the particle count test according to respective sop. Perform the test at rest and in operation conditions for 3 consecutive working days at each condition. Record the results in appendix – 1.
    =>Test instrument id:
    =>Acceptance criteria: the particle count specifications under the laminar air flow unit should meet the specification of room class a.

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Microbial Count test

  • Purpose: To ensure that the microbial count meets the specification of clean room class A.
  • Test Method: Perform the test at rest and in operation conditions for 3 consecutive working days at each condition. All results must be recorded on the appropriate forms of monitoring methodology and be compared with the alarm and specified action limits. The limits of sterile and non-sterile applications are specified below. Details are to be recorded in Appendix – 2.
  • Test Instrument ID: N/A
  • Acceptance Criteria: The microbial count must meet the clean room class – A specifications.

Sl. No.Room ClassAir Sample
(cfu/m3)
Settle Plates
(cfu/4 hours)
Contact Plate (cfu/plate)
01A<1<1<1
02B1055

Deviation And Failure Investigation Summary

Record in the column below any deviations or failures that occurred during the PQ exercise.

Deviation/ FailureDeviation/ FailureDescription and assessment of impact on validationInitialDate
1
2
3

Reference Documents

Sl. No.Title of the DocumentDocument No.
01Clean rooms and associated controlled environments part 1: classification of air cleanlinessISO 14644 – 1
02Clean rooms and associated controlled environments Part 3: Test methodsISO 14644 – 2
03Standard Operating Procedure For
Environmental Monitoring (Microbial & Particle Count)
In-house SOP

Conclusion

  • The performance qualification complies / does not comply with the acceptance criteria taken above and the obtained results are attached with the protocol.
  • The laminar air flow unit of sterile production area of cephalosporin block is / is not qualifying the performance qualification test as per this protocol.
  • Hence, the system can be / cannot be used for normal operation.

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Laminar Air Flow Performance Qualification Protocol

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Laminar Air Flow Operational Qualification

Laminar Air Flow Operational Qualification, Purpose

Laminar Air Flow Operational Qualification, To authenticate and document that the Laminar Air Flow Unit of Sterile production area of Cephalosporin Block of XX Pharmaceuticals Limited (XPL) operates as designed and intended. The operational parameters should be consistent with the requirements for the manufacturing process.

Laminar Air Flow Operational Qualification, Scope

This Operational Qualification (OQ) is to be performed against agreed acceptance criteria on the Laminar Air Flow Unit installed above the turn table after Depyrogenation Tunnel in Room of Sterile Production floor of Cephalosporin Block. The scope will include the verification of previously calibrated instruments and the availability of operational, maintenance and cleaning procedures.

Responsibility

  • Preparation of protocol
    -Engineering Department.
  • Executing the protocol
    -Engineering department or other suitably qualified staff allocated from the site or contracted specialists as appropriate.
  • Providing documentation of the equipment
    -Related departments i.e. Engineering Production, Validation or other appropriate departments and manufacturer.
  • Data documentation and preparation of the report
    -Raw data documentation is the responsibility of those conducting the validation. Preparation of the report and incorporation of data will be the responsibility of the Engineering Department.

Background

Project Description & Related issues
[][]This Laminar Air Flow Unit has been installed in the year 20xx in the sterile production area of Cephalosporin Block for dispensing of raw materials.

Purchasing Information

[][]Manufacturer: GUSU
[][]Local Agent: LS Techno Trade
[][]Model Number: GMP Standard

System Description

[][]A Laminar Air Flow Unit is used to provide unidirectional air flow. Grade A is ensured under LAF using H14 HEPA filter.

[][]A blower is used to circulate the air. The air flow direction of this LAF is vertical.

[][]There is a differential pressure gauge to see the differential pressure across the filters which indicate the blockage of filters.

Verification of Sops

[][]The standard operating procedure for operation and cleaning procedure of Laminar Air Flow Unit must be at least in draft format before commencement of OQ. The necessary information are recorded in the following table:

Sl. No.SOP NameEffective DateAcceptable
(Yes/ No)
Initials with date
01Operation and Cleaning of Laminar Air Flow Unit.
02

Verification of Training

  • Training should have been given regarding operation, maintenance and cleaning of Laminar Air Flow Unit to all operators and must be documented.
  • The documentation status is to be recorded in the following tables.
  • The training is to be recorded in the following tables.

Trainer NameTraining course/SOP NameTrainee NameInitialsDate

Operational testing

  • Verification of start-up and normal sequence of operation
  • Record results of checks in the following table:

Criterion No.Action to be takenAcceptance CriteriaCompliant
Yes/No
Initial/Date
01Switch ON the main power.The voltage will be shown on the display.
02Switch ON the blower by pressing the ON button.The blower has to be ON and air will start flowing vertically.
03Differential Pressure gauge for HEPA Filter is functioning properly.The differential pressure gauge should show pressure from zero to positive direction.

Smoke Test

  • Purpose: The purpose of the smoke test is to justify the airflow direction under the HEPA Filter is unidirectional.
  • Test Method:
  • The smoke test will be performed by ______________________ According to the SOP No. _____________________________.
    Test Equipment/Material: Dry Ice and water.
  • Acceptance Criteria: Airflow direction is unidirectional and vertical. Attach a copy of the report of Smoke test.

Filter Integrity Test

  • Purpose: Filter Integrity test is performed using Poly Alpha Olephin (PAO) to check any leakage of the filter.
  • Test Method: The test is performed by ____________________ according to the SOP NO. ______________________________.
  • Test Equipment: PAO generator, Optical Photometer.
  • Acceptance Criteria: Any leakage greater than 0.01% of the upstream challenge aerosol concentration is considered unacceptable and wants repairs and resetting.
  • Attach a copy of HEPA filter integrity test certificate in attachment.

Air Velocity Test

  • Purpose: The purpose of this test is to ensure the air velocity for laminar flow.

    Test Method:

  • Measure the air velocity under each HEPA filter of the equipment at the distance of 6” below the filter grill and record the velocity at 5 locations (Shown in following figure) and take the average reading with the anemometer.
  • Measure the air velocity under each HEPA filter of the equipment at the operation level and record the velocity at 5 locations (Shown in following figure) and take the average reading with the anemometer.
  • Test Equipment: Calibrated anemometer.
  • Acceptance Criteria: Measured air velocity should be in the range of 0.36 m/sec ~ 0.54 m/sec.
    Result:

No. 1 HEPA

No. 2 HEPA result shall be plotted.

Conclusion

[][]The Operational Qualification of Laminar Air Flow Unit of Cephalosporin Block Complies / Does Not Comply with the acceptance criteria taken above and the results obtained have been filled up in the respective table of each test.
[][]The Laminar Air Flow Unit of Cephalosporin Block is / is not qualifying the Operational Qualification test as per this Protocol. Hence, the system can be / cannot be used for Performance Qualification.

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Laminar Air Flow Operational Qualification

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Laminar Air Flow Installation Qualification

Laminar Air Flow Installation Qualification, Purpose

Laminar Air Flow Installation Qualification, To verify and document that the Laminar Air Flow Unit of Sterile Area of Cephalosporin Block is designed, built and installed according to XX Pharmaceuticals Limited’s demand and manufacturer specifications and that documentation is compiled to verify the integrity of the installation.

Laminar Air Flow Installation Qualification, Scope

This Installation Qualification (IQ) is to be performed against agreed acceptance criteria on the Laminar Air Flow Unit installed above the turn table after Depyrogenation Tunnel in Room of Sterile Production floor of Cephalosporin Block. The scope will include assessment of equipment design and installation, connection of utilities, presence and function of instruments.

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Responsibilities

[][]Preparation of protocol
=>Engineering Department.
[][]Executing the protocol
=>Engineering department or other suitably qualified staff allocated from the site or contracted specialists as appropriate.
[][]Providing documentation on the equipment
=>Related departments i.e. Engineering Production, Validation or other appropriate departments and manufacturer
[][]Data documentation and preparation of the report
=>Raw data documentation is the responsibility of those conducting the validation. Preparation of the report and incorporation of data will be the responsibility of the Engineering Department.

Background

[][]Project Description & Related issues
=>This Laminar Air Flow Unit has been installed in the year 20xx in the sterile production area of Cephalosporin Block for dispensing of raw materials.

Purchasing Information

Item/ Brand

=>Manufacturer: GUSU
=>Local Agent: Precisa Techno Trade
=>Model Number: Standard

System Description

A Laminar Air Flow Unit is used to provide unidirectional air flow. Grade A is ensured under LAF using H14 HEPA filter. A blower is used to circulate the air. The air flow direction of this LAF is vertical. There is a differential pressure gauge to see the differential pressure across the filters which indicate the blockage of filters.

Following Document Shall be noted:
[][]Verification of Installation
[][]Design Documentation
=>General Characteristics
=>Engineering Specifications
[][]Instruments verification
[][]Utility Verification
[][]Deviation and Failure Investigation Summary

Conclusion

[][]The Installation Qualification of Laminar Air Flow Unit of Cephalosporin Block Complies / Does Not Comply with the acceptance criteria taken above and the results obtained have been filled up in the respective table of each test.
[][]The Laminar Air Flow Unit of Cephalosporin Block is / is not qualifying the Installation Qualification test as per this Protocol. Hence, the system can be / cannot be used for Operational Qualification.

Download Here

Laminar Air Flow Installation Qualification

Laminar Air Flow Installation Qualification Read More »